Staining:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
Multiplex Assay:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
Polymerase Chain Reaction:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
CRISPR:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
Knock-Out:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
Non-Homologous End Joining:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
Activity Assay:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
Modification:Article Title: CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Article Snippet: .. Genus Bacterial Strains Gram Staining Targeted Gene/s Resulted Modifications/Outcomes References A. baumannii gltA and β-lactamase genes Multiplex PCR and CRISPR-Cas12a [265] A. baumannii AYE pyrF Genome editing, gene knock-out, and gene manipulation and deletions [266] Actinoplanes Gram + Actinoplanes sp. SE50/110 MelC Genome editing and gene deletions [267] Bacillus Gram + B. subtilis ku and ligD Genome alteration, DSB, and non-homologous end-joining (NHEJ) repair [268] B. subtilis uppS CRISPRi and gene activity of essential genes [269] B. subtilis ATCC 6051a amyE, aprE, nprE, spoIIAC, and srfC Genome editing and gene manipulation (up to 50%) [270] B. subtilis 168 trpc2 Genome alteration, gene deletions, and point mutations [271] B. smithii pyrF Genome modification, gene deletions, and silencing and insertions (90%, 100%, and 20%, respectively) [272] B. smithii ET 138 ldhL Genome editing, gene inactivation, and silencing with ThermoCas9 (active @ 55 ◦C) [273] B. licheniformis yvmC Genome editing and gene knock-outs and integration [274] Brucella Gram – B. melitensis BE3 Gene manipulation and 100% base replacement (C-T) [275] Campylobacter Gram – C. jejuni strains M1Cam and 81–176 flaA, flab, astA, and flgR, CRISPRi-based repression [257] C. jejuni strains M1Cam and 81–176 flaA, flab, and flgR, CRISPRi-based gene repression [258] Caulobacter Gram – C. crescentus ctrA and gcrA CRISPRi and gene knock-downs [256] Clostridium Gram + C. acetobutylicum ATCC 824 upp Genome editing and gene deletions, substitution, and insertions [276] C. acetobutylicum DSM792 hprK Genome editing and gene deletion and modifications [277] C. autoethanogenum adh and 2,3-bdh Genome editing and gene deletions [278] C. acetobutylicum ATCC 824 and C. beijerinckii NCIMB 8052 spoOA CRISPRi and genome deletion (C. acetobutylicum = 20 bp) (C. beijerinckii = 20–1149 bp) [279] Table 2. ..
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